CrkL antibody (C-Term)

Catalog No. ABIN121674

Product Details anti-CrkL Antibody

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB)

Purification: Affinity chromatography

Immunogen: Synthetic peptide sequence KIFDPQNPDENE derived from C-terminus of CRKL

Application Details

Application Notes: Western Blot: 1/200 - 1/500, this antibody detects a band of approximately 33 kDa in mouse kidney cell lysates, overnight incubation with primary antibody is recommended.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: TRIS buffered saline pH 7.3 containing 0.02 % Sodium Azide and 0.5 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL (CRKL Products)

Synonyms: CRKL antibody, wu:fa55g05 antibody, wu:fj60e01 antibody, zgc:63469 antibody, 1110025F07Rik antibody, AA589403 antibody, AI325100 antibody, Crkol antibody, CRK like proto-oncogene, adaptor protein antibody, v-crk avian sarcoma virus CT10 oncogene homolog-like antibody, CRKL antibody, crkl antibody, Crkl antibody

Background: CRKL is a 33 kDa adaptor protein which has been shown to activate the RAS and JUN kinase signalling pathways and transforms fibroblasts in a RAS-dependant manner. CRKL is a substitute of BCR-ABL tyrosine kinase.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor