CCR8
Reactivity: Human
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunohistochemistry with formalin fixed paraffin embedded tissues: 28 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.7, containing 0.01 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-80 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
CCR8, a Chemokine Receptor, binds the CC-chemokine I-309, which is secreted by umbilical vein endothelial cells. CCR8 also binds viral monocyte inflammatory protein-I (vMIP-I), which is induced by Kaposi sarcoma-related human herpesvirus-8. CCR8 induces endothelial chemotaxis and may function as an HIV-1 co-receptor on primary human cells, contributing to HIV-1-induced thymic pathogenesis. CCR8 expression has been reported in spleen and thymus, natural-killer cells, monocytes, and T cells, and at lower levels in peripheral blood leukocytes. Increased expression has been seen in allergen-challenged atopic asthma. No CCR8 expression was found in mesangial cells of the kidney cortex. ESTs have been isolated from human normal kidney, eye, and B cell/lung/testis libraries and from human brain, germ cell, and pancreas cancer libraries.Synonyms: C-C CKR-8, C-C chemokine receptor type 8, CC-CKR-8, CC-chemokine receptor CHEMR1, CCR-8, CKRL1, CMKBR8, CMKBRL2, Chemokine receptor-like 1, GPR-CY6, TER1