CDC7 antibody (Kinase Domain)

Catalog No. ABIN122614

Product Details anti-CDC7 Antibody

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Binding Specificity: Kinase Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC7 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reacts with CDC7-Related Protein Kinase (CDC7L1) (family: Protein Kinase, subfamily: CDC7)

Characteristics: Synonyms: CDC7L1, CDC7-related kinase, HsCdc7, huCdc7, Cell division cycle 7-related protein kinase

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugatedEpitope: Kinase domain of humanGenename: CDC7Remarks: The immunizing peptide shows 63 % identity (79 % similarity) with mouse.

Application Details

Application Notes: Immunohistochemistry on paraffin embedded sections: 5,2 μg/mLntigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20 min. Remove fromheat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CDC7

Target: CDC7 (Cell Division Cycle 7 (CDC7))

Alternative Name: CDC7 (CDC7 Products)

Synonyms: CDC7L1 antibody, HsCDC7 antibody, Hsk1 antibody, huCDC7 antibody, AI597260 antibody, Cdc7l1 antibody, muCdc7 antibody, cell division cycle 7 antibody, cell division cycle 7-related protein kinase antibody, cell division cycle 7 (S. cerevisiae) antibody, CDC7 antibody, Tsp_08585 antibody, Cdc7 antibody

Background: CDC7L1, CDC7 type protein kinase, regulates firing of the origins of initiation in DNA replication. CDC7L1 forms a complex with a Dbf4-related regulatory subunit to generate an active kinase. Mini-chromosome maintenance protein (MCM) is the major physiological target of Cdc7L1. At least three mRNA variants have been reported. Overexpression of the CDC7L1 protein may be associated with neoplastic transformation for some tumors. CDC7L1 has been documented in a wide range of tissues. One variant is expressed ubiquitously in human tissues while at least one other has been shown to be testis-specific. ESTs have been isolated from several tissue libraries, including normal embryo, gallbladder, lymph node and thymus and cancerous duodenum and embryo.Synonyms: CDC7-related kinase, CDC7L1, Cell division cycle 7-related protein kinase, HsCdc7, huCdc7

Gene ID: 8317

UniProt: O00311

Pathways: Mitotic G1-G1/S Phases, DNA Replication