CD18 antibody (APC)

Antigen: CD18

Clonality: Monoclonal (MEM-48)

Host: Mouse

Reactivity: Human

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN125688

Additional Information

Immunogen: Leukocytes of a patient suffering from a LGL-type leukemia.

Isotype: IgG1

Clone: MEM-48

Description: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Specificity: The antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-richrepeat 3 of the CD18 antigen (integrin beta2 subunit, beta2 integrin). CD18 is a 90-95 kDatype I transmembrane protein expressed on all leukocytes. This antibody does not react with: Dog (Canine) .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with cross-linked Allophyc

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western Blotting analysis (non-reducing conditions) of whole cell lysate using anti-human CD18 (MEM-48). Lane 1: KG-1a human acute myeloid leukemia cell line. Lane 2: HPB-ALL human peripheral blood T cell leukemia cell line

Surface staining of human peripheral blood cells with anti-human CD18 (MEM-48) PE. Cells in the granulocyte gate were used for analysis.

Publications

Publications: Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

Garnotel, Rittiue, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Nakayama, Yoshizaki, Prinetti et al.: "Lyn-coupled LacCer-enriched lipid rafts are required for CD11b/CD18-mediated neutrophil phagocytosis of nonopsonized microorganisms." in: Journal of leukocyte biology, Vol. 83, Issue 3, pp. 728-41, 2008 (PubMed).

Sewald, Gebert-Vogl, Prassl et al.: "Integrin subunit CD18 Is the T-lymphocyte receptor for the Helicobacter pylori vacuolating cytotoxin." in: Cell host & microbe, Vol. 3, Issue 1, pp. 20-9, 2008 (PubMed).

Zemani, Silvestre, Fauvel-Lafeve et al.: "Ex vivo priming of endothelial progenitor cells with SDF-1 before transplantation could increase their proangiogenic potential." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 28, Issue 4, pp. 644-50, 2008 (PubMed).

Morova, Osicka, Masin et al.: "RTX cytotoxins recognize beta2 integrin receptors through N-linked oligosaccharides." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 14, pp. 5355-60, 2008 (PubMed).

Cera, Fabbri, Molendini et al.: "JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling." in: Journal of cell science, Vol. 122, Issue Pt 2, pp. 268-77, 2009 (PubMed).

Tran, Glass, Uzel et al.: "Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 5, pp. 2929-38, 2009 (PubMed).

Balashova, Shah, Patel et al.: "Aggregatibacter actinomycetemcomitans LtxC is required for leukotoxin activity and initial interaction between toxin and host cells." in: Gene, Vol. 443, Issue 1-2, pp. 42-7, 2009 (PubMed).