CD18 antibody (APC)

Antigen: CD18

Clonality: Monoclonal (MEM-148)

Host: Mouse

Reactivity: Human

Conjugate: APC

Application: Flow Cytometry (FACS)

Catalog no.: ABIN125714

Additional Information

Immunogen: Peripheral blood mononuclear cells

Isotype: IgG1

Clone: MEM-148

Description: CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule toconstitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2(CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18).In most cases, the response mediated by the integrin is a composite of the functions of itsindividual subunits. These integrins are essential for proper leukocyte migration, mediatingintercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severereduction of CD18 expression leads to the development of a psoriasiform skin disease.CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient tomediate leukotoxin-mediated cytolysis.

Specificity: The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible inintact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa typeI transmembrane protein expressed on all leukocytes.HLDA VI, WS Code AS A052 This antibody does not react with: Porcine .

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. Preparation: The purified antibody is conjugated with cross-linked Allophyc

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).