Basic Cytokeratin antibody

Details for Product No. ABIN125988
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Antigen
Reactivity
Amphibian, Cow (Bovine), Human
(6), (6), (5), (3), (3), (3), (2), (2), (2)
Host
Mouse
(6)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(6), (5), (4), (4), (2), (2), (1)
Pubmed 5 references available
Quantity 50 µg
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Catalog No. ABIN125988
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Immunogen Cytoskeletal proteins from cultured Human MCF-7 cells
Clone Ks pan 1-8
Isotype IgG2a
Specificity This antibody represents an excellent marker for distinguishing carcinomas from non- epithelial tumors. Polypeptides Reacting: Mr 52 500 - Mr 68 000 keratins (type II keratins K1-K8, formerly also designated cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: all epithelium-derived neoplasms. Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29 Detroit 562, RPMI 2650, SSC-12 Bovine BMGE+H, BMGE-H, MDBK
Purification Affinity Chromatography on Protein A
Background This antibody represents an excellent marker for distinguishing carcinomas fromnon-epithelial tumors.
Application Notes Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water
Buffer Final solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Achtstätter, Fouquet, Rungger-Brändle et al.: "Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species." in: Differentiation; research in biological diversity, Vol. 40, Issue 2, pp. 129-49, 1989 (PubMed).

General Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Gould, Koukoulis, Jansson et al.: "Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1143-55, 1990 (PubMed).

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

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