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Details for Product No. ABIN125988

Basic Cytokeratin antibody

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Antigen
Reactivity
»Alternatives Amphibian, Cow (Bovine), Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN125988
Quantity 50 µg
Price
401.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Cytoskeletal proteins from cultured Human MCF-7 cells
Clone Ks pan 1-8
Isotype IgG2a
Specificity This antibody represents an excellent marker for distinguishing carcinomas from non- epithelial tumors. Polypeptides Reacting: Mr 52 500 - Mr 68 000 keratins (type II keratins K1-K8, formerly also designated cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: all epithelium-derived neoplasms. Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29 Detroit 562, RPMI 2650, SSC-12 Bovine BMGE+H, BMGE-H, MDBK
Purification Affinity Chromatography on Protein A
Background This antibody represents an excellent marker for distinguishing carcinomas fromnon-epithelial tumors.
Application Notes Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore in 1 mL dist. water
Buffer Final solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
Preservative Sodium azide
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8°C. DO NOT FREEZE!
Expiry Date 12 months
Background publications Achtstätter, Fouquet, Rungger-Brändle et al.: "Cytokeratin filaments and desmosomes in the epithelioid cells of the perineurial and arachnoidal sheaths of some vertebrate species." in: Differentiation; research in biological diversity, Vol. 40, Issue 2, pp. 129-49, 1989 (PubMed).

Moll, Schiller, Franke: "Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns." in: The Journal of cell biology, Vol. 111, Issue 2, pp. 567-80, 1990 (PubMed).

Gould, Koukoulis, Jansson et al.: "Coexpression patterns of vimentin and glial filament protein with cytokeratins in the normal, hyperplastic, and neoplastic breast." in: The American journal of pathology, Vol. 137, Issue 5, pp. 1143-55, 1990 (PubMed).

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Alternatives for antigen "Basic Cytokeratin", type "Antibodies"
Hosts (7)
Reactivities (7), (6), (5), (4), (3), (3), (3), (2), (2), (1)
Applications (6), (6), (4), (4), (2), (2), (1)
Conjugates (1), (1)
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