Keratin Basic antibody

Catalog No. ABIN4888493

Product Details

Target: Keratin Basic

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Chicken, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Mouse monoclonal antibody AE3 recognizes all basic type cytokeratins (intracellular antigens), namely K1-K8 (58-67 kDa). This antibody reacts with all layers of epidermis (basal layer and above) and is an excellent marker for distinguishing carcinomas from non-epithelial tumours.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rat, Bovine, Rabbit, Chicken

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human epidermal keratins

Clone: AE3

Isotype: IgG1

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: Keratin Basic

Alternative Name: Basic Cytokeratins (Keratin Basic Products)

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,Type II cytokeratins