GSK3 alpha antibody (pSer21)

Catalog No. ABIN126804

Product Details anti-GSK3 alpha Antibody

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Binding Specificity: pSer21

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody specifically recognizes GSK3alpha phosphorylated at serine 21 (Western blot: 51 kDa) but does not crossreact with the homologous phosphorylation site in GSK3beta (phospho-Ser 9).

Characteristics: Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH.

Clone: 9B8

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.0 5g/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3 alpha (GSK3a Products)

Synonyms: 2700086H06Rik antibody, GSK3A antibody, GSK-3[a] antibody, GSK-3[b] antibody, GSK3 antibody, gsk3a antibody, xgsk3 antibody, glycogen synthase kinase 3 alpha antibody, glycogen synthase kinase 3 alpha b antibody, glycogen synthase kinase 3 alpha S homeolog antibody, GSK3A antibody, Gsk3a antibody, gsk3a antibody, gsk3ab antibody, gsk3a.S antibody, Bm1_31380 antibody

Background: Glycogen Synthase Kinase 3 (GSK3) exists in two isoforms, alpha and beta (GSK3alpha, GSK3beta). GSK3 is an active kinase that becomes phosphorylated by PKB/akt upon growth factor stimulation. Phosphorylation of GSK3alpha at serine 21 or GSK3beta at serine 9, respectively, leads to inhibition of kinase activity of the GSK3.Synonyms: Factor A, GSK-3 alpha, GSK3A, Glycogen synthase kinase-3 alpha

Gene ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect