Caspase 9 antibody

Catalog No. ABIN127091

The Rabbit Polyclonal anti-Caspase 9 antibody is suitable to detect Caspase 9 in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for Caspase 9 antibody (ABIN127091)

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 9 Antibody

Specificity: The antibody detects 45 kDa procaspase-9, 35/37 kDa cleaved large fragment and the 25 kDa fully cleaved Caspase-9.

Purification: Protein A Chromatography.

Immunogen: Synthetic peptide surrounding amino acid 345 of Mouse Caspase-9

Application Details

Application Notes: Western Blot: 0.5-4.0 μg/mL. Mouse small intestine and rat kidney tissue lysates can be used as Positive Controls.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage.

Target Details for Caspase 9

Target: Caspase 9 (CASP9) (Caspase 9, Apoptosis-Related Cysteine Peptidase (CASP9))

Alternative Name: Caspase-9

Background: Caspase-9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase- 9/Apaf-1. The complex processes procaspase-9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase-9 further cleaves other caspase members including caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase-9 can also be cleaved in vivo by caspase-3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.Synonyms: APAF-3, APAF3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, CASP9, ICE-LAP6, ICE-like apoptotic protease 6, MCH6

Gene ID: 842

NCBI Accession: NP_001220

UniProt: P55211

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Caspase Cascade in Apoptosis, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Endopeptidase Activity