Green Fluorescent Protein (GFP) antibody (Peroxidase)

Antigen: Green Fluorescent Protein (GFP)

Clonality: Polyclonal

Host: Mouse

Conjugate: Peroxidase (POD)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN129566

Additional Information

Immunogen: The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Format: Lyophilized

Application Details

Application Notes: Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Monoclonal and polyclonal forms of anti-GFP assayed by ELISA for direct binding of antigen recognize wild type, recombinant and enhanced forms of GFP. Monoclonal and polyclonal forms anti-GFP assayed in a sandwich ELISA are well suited to titrate GFP in solution using either form of the antibody as the capture or detection antibodies. The detection antibody is typically conjugated to biotin and complexed with streptavidin-HRP (code S000-03). Fluorochrome conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone. Peroxidase conjugated anti-GFP assayed by immunoblot shows a 42 kDa band when reacted with GFP on a western blot. Optimal titers for applications should be determined by the researcher.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Gentamicin Sulfate. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Tags/Labels

Restrictions: For Research Use only