V5 Epitope Tag antibody

Details for Product No. ABIN129577, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Rabbit
221
134
37
9
5
4
3
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
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27
19
11
10
10
9
9
9
8
8
8
8
8
8
7
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6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
342
265
123
91
71
65
53
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.
Sequence GKPIPNPLLG LDST
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Alternative Name V5-Tag
Target Type Tag
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG, HA and V5. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.
Gene ID 55775699
Research Area Tags/Labels
Application Notes Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.
Restrictions For Research Use only
Format Liquid
Concentration 0.73 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (ABIN129577) Anti-V5 epitope tag polyclonal antibody detects V5-tagged recombinant protein by west...