Anti-rat T cytotoxic/suppressor cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells, the majority of NK cells, and the granular intraepithelial leukocytes in the small intestine (1,2,3,4). The antigen recognized is a complex of surface glycoproteins of Mr 34, 39, and 76 kDa and is the rat homologue of the human CD8 and the mouse Ly 2.3 antigen (5,8). ABIN133185labels all peripheral T cells that are unlabelled by the ABIN133185(W3/25) monoclonal antibody. It labels a T cell subset which mediates the suppression of antibody formation (1) and the cytotoxic cell precursor (2). ABIN133185and ABIN133185can be used together to fractionate T cells by sorting in FACS or by rosette depletion (1,2) or can be used together to study subsets of T cells in the rat which mediate lethal graft versus host disease (7). This antibody is one of the 3 monoclonal antibodies which labels T lymphocyte populations in the rat, these being ABIN133185(W3/13) which labels all T cells, as well as ABIN133185(W3/25) and ABIN133185(MRC OX-8) which label non-overlapping T cell populations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (9).