B-Cell CLL/lymphoma 2 (BCL2) antibody

Antigen: B-Cell CLL/lymphoma 2 (BCL2)

Synonyms: Bcl-2, bcl-2, BCL2

Clonality: Monoclonal (10C2)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN135029

Additional Information

Alternative name: Bcl-2

Immunogen: KLH-conjugated peptide corresponding to 61-76 amino acid sequence of murine Bcl-21 Ig

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: 10C2

Description: Mouse and rat Bcl-2 (Mr 26 kDa) Apoptosis, or programmed cell death, is a well-documented phenomenon in many cellular systems.2 It plays a key role in tissue and organ development as well as in adult tissues during cell turnover. Apoptosis can be induced by a variety of internal and external stimuli including growth factor deprivation, cytokine treatment, antigen-receptor engagement, cell-cell interactions, irradiation and glucocorticoid treatment.3 Bcl-2 is a widely studied protein that has been shown to be a potent inhibitor of programmed cell death. It has been localized to the outer mitochondrial membrane, perinuclear membrane, and endoplasmic reticulum. Bcl-2 is expressed in memory and resting, or other long-lived lymphoid cells, follicular mantle cells, medullary thymocytes, and lymphomas. Germical center cells and cortical thymocytes are negative for Bcl-2. Upregulation of Bcl-2 prevents or delays apoptosis induced by a variety of stimuli, including growth factor deprivation, -irradiation, glucocorticoids, and chemotherapeutic agents. During lymphoid development, expression of the Bcl-2 protein appears to be regulated in a stage-specific manner, and is thought to be a survival signal for positive selection. The monoclonal antibody 10C2 reacts with both mouse and rat Bcl-2.

Application Details

Application Notes: WORKING DILUTIONS Western Blotting:Purified antibody 1 ug/mL Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Storage: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The products should be stored at 2-8C. Each product is stable for the period shown on the label if stored as directed.

Research Area: Cancer, Apoptosis/Necrosis

Restrictions: For Research Use only

Publications

Publications: Cohen: "Programmed cell death in the immune system." in: Advances in immunology, Vol. 50, pp. 55-85, 1991 (PubMed).

Rajah, Bhala, Nunn et al.: "7S nerve growth factor is an insulin-like growth factor-binding protein protease." in: Endocrinology, Vol. 137, Issue 7, pp. 2676-82, 1996 (PubMed).

Hsu, Youle: "Nonionic detergents induce dimerization among members of the Bcl-2 family." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13829-34, 1997 (PubMed).