CD40 Ligand (CD40LG) antibody

Details for Product No. ABIN135215
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Antigen
Synonyms CD154, cd40l, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40-L, Cd40l, Ly-62, Ly62, Tnfsf5
Reactivity
Mouse (Murine)
(238), (130), (32), (9), (5), (3), (3), (1)
Host
Hamster
(128), (86), (81), (17), (13), (12)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(45), (33), (17), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
(191), (128), (92), (39), (34), (33), (25), (21), (18), (10), (6), (5), (4), (3), (3), (3), (1)
Pubmed 7 references available
Quantity 0.5 mg
Options
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Catalog No. ABIN135215
244.20 $
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Immunogen Activated mouse Th1 clone D1.6 1
Clone MR1
Isotype IgG
Characteristics The antibody detects: CD154 (Mouse CD40 ligand/gp39), Mr 39 kDa
The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens, yet does not alter the immune response to TI antigens.
Purification Purified
Alternative Name CD154/CD40L
Background CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporin-sensitive.
Application Notes Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro blockage of CD154 function

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0.
Preservative Azide free
Handling Advice Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
General Armitage, Fanslow, Strockbine et al.: "Molecular and biological characterization of a murine ligand for CD40." in: Nature, Vol. 357, Issue 6373, pp. 80-2, 1992 (PubMed).

Noelle, Roy, Shepherd et al.: "A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 14, pp. 6550-4, 1992 (PubMed).

Hollenbaugh, Grosmaire, Kullas et al.: "The human T cell antigen gp39, a member of the TNF gene family, is a ligand for the CD40 receptor: expression of a soluble form of gp39 with B cell co-stimulatory activity." in: The EMBO journal, Vol. 11, Issue 12, pp. 4313-21, 1992 (PubMed).

Foy, Page, Waldschmidt et al.: "An essential role for gp39, the ligand for CD40, in thymic selection." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1377-88, 1995 (PubMed).

Aruffo, Farrington, Hollenbaugh et al.: "The CD40 ligand, gp39, is defective in activated T cells from patients with X-linked hyper-IgM syndrome." in: Cell, Vol. 72, Issue 2, pp. 291-300, 1993 (PubMed).

Foy, Shepherd, Durie et al.: "In vivo CD40-gp39 interactions are essential for thymus-dependent humoral immunity. II. Prolonged suppression of the humoral immune response by an antibody to the ligand for CD40, gp39." in: The Journal of experimental medicine, Vol. 178, Issue 5, pp. 1567-75, 1993 (PubMed).

Roy, Waldschmidt, Aruffo et al.: "The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 5, pp. 2497-510, 1993 (PubMed).

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