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L-Selectin antibody (Selectin L) (PE)

Details for Product anti-SELL Antibody No. ABIN135252, Supplier: Log in to see
Antigen
  • A.11
  • AI528707
  • CD62L
  • L-selectin
  • LAM1
  • LECAM-1
  • LECAM1
  • LEU8
  • Lnhr
  • LNHR
  • LSEL
  • Ly-22
  • Ly-m22
  • Lyam-1
  • Lyam1
  • LYAM1
  • PLNHR
  • SELE
  • TQ1
Reactivity
Mouse (Murine)
527
87
73
23
10
1
1
1
Host
Rat
361
252
82
15
2
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This L-Selectin antibody is conjugated to PE
98
84
42
32
15
14
14
14
13
11
10
10
8
8
8
8
8
8
8
7
7
6
4
4
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
601
188
156
154
89
76
60
47
21
15
14
9
6
5
5
3
2
2
2
2
2
2
2
2
1
1
1
Supplier
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Immunogen C3H/eb cloned mouse B lymphoma 38C-13 1
Clone MEL-14
Isotype IgG2a
Characteristics The antibody detects: CD62L/L-selectin, Mr 90 kDa
The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.
Purification boric acid precipitation
Alternative Name CD62L/L-selectin (SELL Antibody Abstract)
Background CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.
Application Notes Working Dilution:
FACS: 01 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

In vivo and in vitro blocking of adhesion, Cytotoxicity studies

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS / NaN3 and a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze!
Protect conjugated products from light.

Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Sprent, Tough: "Lymphocyte life-span and memory." in: Science (New York, N.Y.), Vol. 265, Issue 5177, pp. 1395-400, 1994

Gallatin, Weissman, Butcher: "A cell-surface molecule involved in organ-specific homing of lymphocytes." in: Nature, Vol. 304, Issue 5921, pp. 30-4, 1983