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NK-1.1 antibody (FITC)

Details for Product No. ABIN135341, Supplier: Log in to see
Antigen
  • AI462337
  • CD161
  • Ly-59
  • Ly55c
  • Ly59
  • NK-RP1
  • NK1.1
  • NKR-P1.9
  • NKRP1
  • NKRP140
  • Nk-1
  • Nk-1.2
  • Nk1
  • Nk1.2
  • Nkrp1c
  • ly-55c
  • killer cell lectin-like receptor subfamily B member 1C
  • Klrb1c
Reactivity
Mouse (Murine)
155
16
Host
Mouse
130
15
11
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
21
18
13
10
6
5
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
127
40
17
14
13
11
10
8
7
5
5
4
2
2
2
2
2
2
Options
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Immunogen CE mouse spleen and bone marrow cells enriched for NK-1+ cells 1
Clone PK136
Isotype IgG2a
Characteristics The antibody detects: Mouse NK1.1 alloantigen, Mr 80-85 kDa (39 kDa under reducing conditions)
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name NK1.1
Background NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB). NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.
Application Notes Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Comment

Induction of proliferation of a subset of NK cells, In vivo depletion of NK1.1 cells, In vitro redirected lysis, In vitro cytotoxicity assays

Sample Volume 1 mL
Restrictions For Research Use only
Buffer Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Storage 4 °C
Background publications Routes, Ryan, Ryan, Nakamura: "MHC class I molecules on adenovirus E1A-expressing tumor cells inhibit NK cell killing but not NK cell-mediated tumor rejection." in: International immunology, Vol. 13, Issue 10, pp. 1301-7, 2001 (PubMed).

Lanier: "Natural killer cells: from no receptors to too many." in: Immunity, Vol. 6, Issue 4, pp. 371-8, 1997 (PubMed).

Michie, Streeter, Butcher, Rouse: "L-selectin and alpha 4 beta 7 integrin homing receptor pathways mediate peripheral lymphocyte traffic to AKR mouse hyperplastic thymus." in: The American journal of pathology, Vol. 147, Issue 2, pp. 412-21, 1995 (PubMed).

Sentman, Hackett, Moore, Tutt, Bennett, Kumar: "Pan natural killer cell monoclonal antibodies and their relationship to the NK1.1 antigen." in: Hybridoma, Vol. 8, Issue 6, pp. 605-14, 1990 (PubMed).