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Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody

Details for Product No. ABIN1449192, Supplier: Log in to see
Antigen
  • ba46
  • edil1
  • oacgd3s
  • MGC75581
  • hst19888
  • MGC145109
  • MFGE8
  • mfge8
  • zgc:154157
  • MGC99228
  • BA46
  • EDIL1
  • HMFG
  • HsT19888
  • MFG-E8
  • MFGM
  • OAcGD3S
  • SED1
  • SPAG10
  • hP47
  • AA408458
  • AI325141
  • Mfgm
  • P47
  • lactadherin
  • AGS
  • MFGME8
  • MFGMP-E8
Reactivity
Mouse (Murine)
99
14
3
3
Host
Hamster
73
35
2
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
10
8
8
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
83
42
29
23
19
15
11
9
8
5
4
2
1
Supplier
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Available images

Immunogen Thioglycollate-elicited mouse peritoneal macrophages
Clone 2422
Isotype IgG
Specificity This antibody reacts with MFG-E8 short and long form.
Cross-Reactivity (Details) Species reactivity (tested):Mouse
Purification Protein A agarose
Alternative Name Lactadherin (MFGE8 Antibody Abstract)
Background Apoptotic cells are rapidly removed by phagocytosis to clear the living cells and tissues of intracellular materials being released from dying cells. Phagocytes recognize apoptotic cells through milk fat globule-EGF-factor 8 (MFG-E8). MFG-E8 is a secreted glycoprotein from thioglycollate-elicited macrophages and bind to phosphatidylserine exposed on the cell surface of apoptotic cells, then bind strongly to cells expressing ανβ3 integrin via its RGD (arginine-glycine-aspartate) motif. Thus, MFG-E8 is an important protein to mediate the engulfment of apoptotic cells by activated macrophages.Synonyms: Breast epithelial antigen BA46, HMFG, MFGE8, MFGM, Milk fat globule-EGF factor 8
Gene ID 4240
NCBI Accession NP_001108086
UniProt Q08431
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Upon receipt, store (in aliqouts) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody (ABIN1449192) anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody
Flow Cytometry (FACS) image for anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody (ABIN1449192) anti-Milk Fat Globule-EGF Factor 8 Protein (MFGE8) antibody (Image 2)
Background publications Montecalvo, Shufesky, Stolz et al.: "Exosomes as a short-range mechanism to spread alloantigen between dendritic cells during T cell allorecognition." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 5, pp. 3081-90, 2008 (PubMed).

Fens, Mastrobattista, de Graaff et al.: "Angiogenic endothelium shows lactadherin-dependent phagocytosis of aged erythrocytes and apoptotic cells." in: Blood, Vol. 111, Issue 9, pp. 4542-50, 2008 (PubMed).

Morelli, Larregina, Shufesky et al.: "Endocytosis, intracellular sorting, and processing of exosomes by dendritic cells." in: Blood, Vol. 104, Issue 10, pp. 3257-66, 2004 (PubMed).

Hanayama, Tanaka, Miyasaka et al.: "Autoimmune disease and impaired uptake of apoptotic cells in MFG-E8-deficient mice." in: Science (New York, N.Y.), Vol. 304, Issue 5674, pp. 1147-50, 2004 (PubMed).