Cytochrome B-245, alpha Polypeptide (CYBA) antibody (PE)

Details for Product No. ABIN1449195, Supplier: Log in to see
Antigen
  • zgc:66077
  • MGC80750
  • CYBA
  • p22-phox
  • p22phox
  • p22-PHOX
  • b558
  • nmf333
  • P22-PHOX
  • cytochrome b-245, alpha polypeptide
  • cytochrome b-245 light chain-like
  • p22-phox
  • cyba
  • CYBA
  • LOC101328418
  • Cyba
Alternatives
anti-Human Cytochrome B-245, alpha Polypeptide antibody for Immunofluorescence (fixed cells)
Reactivity
Human, Monkey
48
23
23
4
2
2
1
1
1
1
Host
Mouse
44
4
Clonality (Clone)
Monoclonal ()
Conjugate
PE
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS)
31
18
16
13
5
4
3
2
1
1
1
Options
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Immunogen Human cytochrome b-rich fraction
Clone 7D5
Isotype IgG1
Specificity This antibody recognizes the extracellular peptide portion of primate gp91phox of the Flavocytochrome b558.
No Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) Species reactivity (tested):Human, Monkey.
Purification Protein A agarose
Alternative Name Cytochrome B-245 Light Chain (CYBA Antibody Abstract)
Background The NADPH oxidase is a multicomponent enzyme that transfers electrons from NADPH to O2 to generate superoxide (O2 -), a key part of the phagocytic or neutrophilic respiratory burst response. Flavocytochrome b558 is the catalytic component of the phagocyte NADPH oxidase. It is a transmembrane heterodimer composed of a large glycoprotein, gp91phox (PHagocyte OXidase) and a smaller protein, p22phox. Upon cell stimulation, flavocytochrome b558 assembles with p67phox, p47phox, and the GTP-binding protein Rac and becomes activated to generate O2 -. Mutations in gp91phox, p22phox, or other components of the NADPH oxidase can result in chronic granulomatous disease, which is associated with significant morbidity and mortality due to a predisposition to recurrent bacterial and fungal infections.Synonyms: CYBA, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox
Gene ID 1535
NCBI Accession NP_000092
UniProt P13498
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Buffer PBS, 0.09 % NaN3, 1 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 2-8 °C.
Supplier Images
Flow Cytometry (FACS) image for anti-Cytochrome B-245, alpha Polypeptide (CYBA) antibody (PE) (ABIN1449195) anti-Cytochrome B-245, alpha Polypeptide (CYBA) antibody (PE)
Background publications Zhu, Marchal, Casbon, Stull, von Löhneysen, Knaus, Jesaitis, McCormick, Nauseef, Dinauer: "Deletion mutagenesis of p22phox subunit of flavocytochrome b558: identification of regions critical for gp91phox maturation and NADPH oxidase activity." in: The Journal of biological chemistry, Vol. 281, Issue 41, pp. 30336-46, 2006 (PubMed).

Li, Grunwald, Mathieu, Morel, Stasia: "Crucial role of two potential cytosolic regions of Nox2, 191TSSTKTIRRS200 and 484DESQANHFAVHHDEEKD500, on NADPH oxidase activation." in: The Journal of biological chemistry, Vol. 280, Issue 15, pp. 14962-73, 2005 (PubMed).

Allen, Beecher, Lynch, Rohner, Wittine: "Helicobacter pylori disrupts NADPH oxidase targeting in human neutrophils to induce extracellular superoxide release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 6, pp. 3658-67, 2005 (PubMed).

Carlyon, Abdel-Latif, Pypaert, Lacy, Fikrig: "Anaplasma phagocytophilum utilizes multiple host evasion mechanisms to thwart NADPH oxidase-mediated killing during neutrophil infection." in: Infection and immunity, Vol. 72, Issue 8, pp. 4772-83, 2004 (PubMed).

Brenner, Whiting-Theobald, Linton, Holmes, Anderson-Cohen, Kelly, Vanin, Pilon, Bodine, Horwitz, Malech: "Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells." in: Blood, Vol. 102, Issue 8, pp. 2789-97, 2003 (PubMed).

Burritt, Foubert, Baniulis, Lord, Taylor, Mills, Baughan, Roos, Parkos, Jesaitis: "Functional epitope on human neutrophil flavocytochrome b558." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6082-9, 2003 (PubMed).

Carlyon, Chan, Galán, Roos, Fikrig: "Repression of rac2 mRNA expression by Anaplasma phagocytophila is essential to the inhibition of superoxide production and bacterial proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 12, pp. 7009-18, 2002 (PubMed).

Morgan, Cherny, Price, Dinauer, DeCoursey: "Absence of proton channels in COS-7 cells expressing functional NADPH oxidase components." in: The Journal of general physiology, Vol. 119, Issue 6, pp. 571-80, 2002 (PubMed).

Kurosaka, Ishida, Yamamura, Takase, Otani, Kumon: "A non-surgical rat model of foreign body-associated urinary tract infection with Pseudomonas aeruginosa." in: Microbiology and immunology, Vol. 45, Issue 1, pp. 9-15, 2001 (PubMed).

Kornberg: "Remembering our teachers." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 3-11, 2001 (PubMed).

Yu, Cross, Zhen, Dinauer: "Functional analysis of NADPH oxidase in granulocytic cells expressing a delta488-497 gp91(phox) deletion mutant." in: Blood, Vol. 94, Issue 7, pp. 2497-504, 1999 (PubMed).

Yu, Quinn, Cross, Dinauer: "Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 14, pp. 7993-8, 1998 (PubMed).

Strader, Gaffney, Sugg, Candelore, Keys, Patchett, Dixon: "Allele-specific activation of genetically engineered receptors." in: The Journal of biological chemistry, Vol. 266, Issue 1, pp. 5-8, 1991 (PubMed).

Verhoeven, Bolscher, Meerhof, van Zwieten, Keijer, Weening, Roos: "Characterization of two monoclonal antibodies against cytochrome b558 of human neutrophils." in: Blood, Vol. 73, Issue 6, pp. 1686-94, 1989 (PubMed).