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Autophagy Related 4D, Cysteine Peptidase (ATG4D) (AA 227-257) antibody

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Antigen
Synonyms APG4-D, APG4D, AUTL4, 9830134P12Rik, Apg4d, Atg4dl, Autl4, apg4d
Epitope
AA 227-257
(32), (15), (15), (13), (13), (13), (11), (8), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(130), (51), (38), (3), (3), (1), (1), (1), (1)
Host
Rabbit
(101), (16), (16), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (7), (7), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(97), (52), (24), (21), (17), (15), (1)
Pubmed 5 references available
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Quantity 0.4 mL
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Availability Will be delivered in 6 to 8 Business Days
Immunogen KLH conjugated synthetic peptide between 227~257 amino acids from the Center of Human APG4D Genename: ATG4D
Isotype Ig
Purification Saturated Ammonium Sulfate precipitation followed by dialysis against PBS
Alternative Name APG4D / ATG4D (ATG4D Antibody Abstract)
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.Synonyms: AUT-like 4 cysteine endopeptidase, AUTL4, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D, Cysteine protease ATG4D
Molecular Weight 52922 Da
Gene ID 84971
NCBI Accession NP_116274
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Autophagy Related 4D, Cysteine Peptidase (ATG4D) (AA 227-257) antibody (ABIN1449634) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-Autophagy Related 4D, Cysteine Peptidase (ATG4D) (AA 227-257) antibody (ABIN1449634) Western blot analysis of ATG4D Antibody  in 293 cell line lysates transiently transfe...
Western Blotting (WB) image for anti-Autophagy Related 4D, Cysteine Peptidase (ATG4D) (AA 227-257) antibody (ABIN1449634) Western blot analysis of ATG4D Antibody in HL-60 cell line lysate.
Western Blotting (WB) image for anti-Autophagy Related 4D, Cysteine Peptidase (ATG4D) (AA 227-257) antibody (ABIN1449634) Western blot analysis of ATG4D Antibody  in mouse kidney tissue lysate.
Background publications Greenberg: "Degrade or die: a dual function for autophagy in the plant immune response." in: Developmental cell, Vol. 8, Issue 6, pp. 799-801, 2005 (PubMed).

Baehrecke: "Autophagy: dual roles in life and death?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 505-10, 2005 (PubMed).

Lum, DeBerardinis, Thompson: "Autophagy in metazoans: cell survival in the land of plenty." in: Nature reviews. Molecular cell biology, Vol. 6, Issue 6, pp. 439-48, 2005 (PubMed).

Levine: "Eating oneself and uninvited guests: autophagy-related pathways in cellular defense." in: Cell, Vol. 120, Issue 2, pp. 159-62, 2005 (PubMed).

Shintani, Klionsky: "Autophagy in health and disease: a double-edged sword." in: Science (New York, N.Y.), Vol. 306, Issue 5698, pp. 990-5, 2004 (PubMed).

Catalog No. ABIN1449634
$401.50
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