PTTG1 antibody (Internal Region)

Catalog No. ABIN4889505

Product Details anti-PTTG1 Antibody

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: Reacts with human 22 kDa securin

Cross-Reactivity: Mouse (Murine)

Cross-Reactivity (Details): May cross react with mouse and other species due to sequence homology.

Purification: Purified (protein A)

Immunogen: Synthetic peptide derived from internal domain of the human Securin

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/1000 - IHC: 1/50 to 1/200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: Securin (PTTG1 Products)

Synonyms: EAP1 antibody, HPTTG antibody, PTTG antibody, TUTR1 antibody, PTTG1 antibody, securin antibody, AW555095 antibody, C87862 antibody, Pttg antibody, Pttg3 antibody, Securin antibody, Protein Cut2 antibody, pituitary tumor-transforming 1 antibody, pituitary tumor-transforming gene 1 antibody, securin, sister chromatid separation inhibitor antibody, PTTG1 antibody, Pttg1 antibody, cut2 antibody

Background: Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.

UniProt: O95997