TEK antibody (Biotin)

Catalog No. ABIN155165

The Mouse Monoclonal anti-TEK antibody has been validated for WB, FACS and EIA. It is suitable to detect TEK in samples from Human.

Quick Overview for TEK antibody (Biotin) (ABIN155165)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: Cl-16

Product Details anti-TEK Antibody (Biotin)

Specificity: The unconjugated monoclonal antibody will detect native human TIE-2 /TEK in ELISA experiments and on the surface of different human cell types.

Purification: Protein G purified

Immunogen: Recombinant human soluble CD202b / TIE-2 protein

Isotype: IgG1

Application Details

Application Notes: Western Blot. ELISA. FACS analysis and cell sorting: 2 - 5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore to a concentration of 0.1-1.0 mg/mL with sterile water. Centrifuge vial prior to opening

Buffer: PBS, 50x BSA, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for two weeks or (in aliquots) at -20 °C for longer.

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: CD202b / TEK

Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations, the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK

Gene ID: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding