V5 Epitope Tag antibody (HRP)

Details for Product No. ABIN1573947, Supplier: Log in to see
Antigen
Reactivity
Tag
404
3
3
3
3
Host
Rabbit
221
134
37
9
5
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
28
27
19
11
10
10
9
9
9
8
8
8
8
8
8
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7
6
5
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
342
265
123
92
71
65
53
36
28
13
9
8
5
4
4
2
2
2
1
1
1
1
Options
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Immunogen V5 epitope tag peptide GKPIPNPLLGLDST conjugated - KLH
Sequence GKPIPNPLLG LDST
Isotype IgG
Specificity Rabbit Anti-V5-tag [HRP] Polyclonal Antibody specifically recognizes C-terminal, N-terminal, and internal V5 tagged fusion proteins.
Purification Immunoaffinity chromatography
Alternative Name V5-Tag
Target Type Tag
Background Rabbit Anti-V5-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Rabbit Anti-V5-tag [HRP] polyclonal antibody is suitable for detecting fusion proteins that contain V5-tags.
Research Area Tags/Labels
Application Notes Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Other applications: user-optimized
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Supplier Images
Western Blotting (WB) image for anti-V5 Epitope Tag antibody (HRP) (ABIN1573947) Lane 1-2: V5-tag fusion protein in Hela cell lysate (~52 kD) Lane 3: Negative Hela ce...
Product cited in: Dahl, Corydon, Ränkel, Nielsen, Füchtbauer, Knudsen: "An eEF1A1 truncation encoded by PTI-1 exerts its oncogenic effect inside the nucleus." in: Cancer cell international, Vol. 14, Issue 1, pp. 17, 2014 (PubMed).

Tahir, Yang, Goltsov, Song, Ren, Wang, Chang, Thompson: "Caveolin-1-LRP6 signaling module stimulates aerobic glycolysis in prostate cancer." in: Cancer research, Vol. 73, Issue 6, pp. 1900-11, 2013 (PubMed).

Zhang, Li, Jaiswal, Bayat, Xiong, Sandoval, Charng, David, Haueter, Yamamoto, Graham, Bellen: "The C8ORF38 homologue Sicily is a cytosolic chaperone for a mitochondrial complex I subunit." in: The Journal of cell biology, Vol. 200, Issue 6, pp. 807-20, 2013 (PubMed).