Nestin antibody

Catalog No. ABIN1580439

Product Details anti-Nestin Antibody

Target: Nestin (NES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purification: ascites fluid

Clone: 4D11

Isotype: IgG1

Application Details

Application Notes: For immunocytochemistry on cells in tissue culture or in tissue sections, try this antibody at about between about 1:500 using fluorescent secondary antibodies. For immunoblotting 1:5,000 is recommended. Expect to see a band at ~240kDa and another much weaker at ~90kDa, apparently a breakdown product of the ~240kDa band.

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4°C short term or -20°C long term.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin (NES Products)

Synonyms: paranemin antibody, NES antibody, AA166324 antibody, C78523 antibody, ESTM46 antibody, nestin antibody, nestin S homeolog antibody, NES antibody, nes.S antibody, Nes antibody, nes antibody

Background: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells, which is the origin of the name nestin. Nestin was originally identified as a result of the production of a series of monoclonal antibodies directed against epitopes expressed on formalin fixed embyro day 15 rat spinal cord. One of these antibodies, called Rat 401, stained fibrous profiles in the developing nervous system, but not in the mature nervous system. By screening bacteriophage expression libraries with Rat 401, Lendahl et al. were able to isolate a cDNA encoding the protein to which Rat 401 antibody bound. The protein proved to be an unusual member of the intermediate filament family, containing an alpha-helical region homologous to that found in keratin and neurofilament subunits. The protein was named Nestin as, in the developing nervous system, it is found in neural stem cells. The Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name for Nestin is NES. The Nestin protein has a very short N-terminal region in front of the alpha-helical region and a very long and repetitive C-terminal region. The molecular weight of human Nestin as determined by SDS-PAGE mobility is about 240 kDa. However the real molecular weight is considerably less than this, at 177 kDa, the disparity being likely due to the highly charged region of the C-terminal segment. Nestin is relatively poorly conserved in protein sequence across species boundaries, so that the mouse and human proteins have an overall identity of only 62%. As a result antibodies to the human protein often fail to recognize the rodent homologue and vice versa. However MCA-4D11 stains both rodent and human Nestin. Antibodies to Nestin are widely used to identify neural stem cells. The HGNC name for this protein is NES.