ELISA: Use at a concentration range of 1-2 µg/mL. Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL. WB analysis mouse hippocampal tissue lysate expressing phospho CREB1. ABIN1685160 was used as primary antibody. IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL. Immunohistochemical analysis of frozen mouse hyppocampal sections expressing CREB1. ABIN1685160 was used as primary antibody, dilution 1:200. Immunohistochemical analysis of FFPE mouse thymocytes expressing phospho CREB-1. ABIN1685160 was used as primary antibody, dilution 1:500.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Buffer
PBS solution at a concentration of 1 mg/mL.
Handling Advice
Avoid repeated freeze/thaw cycles.
Storage
-20 °C
Storage Comment
Recombinant IL-21R, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.
Expiry Date
12 months
Target
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
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Background
The IL-21 receptor (IL-21R) is structurally related to IL-2R and IL-15R. In addition, these receptors, together with IL-4R, IL-7R, and IL-9R, share a common gamma -chain. IL-21R appears in T, natural killer (NK), B, and dendritic cells (DC), and it plays an important role in the regulation and function of these cells. IL-21 has, however, an effect on NK cells depending on their activation state. It enhances the proliferation and cytotoxic activity of activated NK cells, but in these cells, IL-21 also promotes apoptosis and thus limits NK cell response. In contrast, IL-21 inhibits IL-15-induced proliferation and maturation of NK cell precursors