FGF1
Reactivity: Human, Mouse, Rat
WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Use at a concentration range of 1-2 µg/mL. Western blot: A suitable range of concentrations of this antibody for WB detection is 10 µg/mL. IHC: A suitable range of concentrations of this antibody for IHC is 10 µg/mL.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
Buffer
PBS solution at a concentration of 1 mg/mL.
Handling Advice
Avoid repeated freeze/thaw cycles.
Storage
-20 °C
Storage Comment
Recombinant CD48, as supplied, can be stored in working aliquots at - 8 °C for one month, or at -20 °C to -70 °C for twelve months.
CD48 is a glycosylphosphatidylinositol-anchored protein belonging to the CD2 subfamily. It is expressed mainly on hematopoietic cells and exists in both membrane-associated and soluble forms. It is a low-affinity ligand for CD2 and is implicated as an important costimulatory molecule in lymphocyte activation. Indeed, CD48 has been described as facilitating cell adhesion, innate responses to bacterial infection, and graft rejection. In addition, CD48 is a high-affinity ligand for B4. CD48/2B4 interactions can modulate T cell, B cell, and natural killer (NK) cell functions and cross-talk. The absence of functional B4/CD48 interactions impairs NK cell cytotoxic response and IFN- release on tumor target exposure. Furthermore, activated NK cells significantly increase the CD3-dependent proliferation of CD8+ and CD4+ T cells by aB4/CD48-dependent mechanism.