CD74
Reactivity: Human
WB, IHC
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only : IHC (5 μg/mL) IP (12 μg/mL) WB (1 μg/mL) A doublet band of ~33-35 kDa is detected corresponding to the apparent molecular mass of the p33 and p35 forms of human CD74.
Positive control: PALA cell lysate.
Restrictions
For Research Use only
Format
Liquid
Buffer
PBS, pH 7.4, 50 % glycerol
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Dilute in PBS or medium that is identical to that used in the assay system.
Storage
-20 °C
Storage Comment
This antibody is stable for at least one year at -20°C. Avoid repeated freezing and thawing.
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Background
CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain. CD74 functions mainly as an MHC class II chaperone which promotes ER exit of MHC class II molecules by directing them to endocytic compartments, preventing peptide binding in the ER, and contributing to peptide "editing" in the MHC class II compartment. CD74 has recently been shown to have a role as an accessory signaling molecule because of its high affinity binding to the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).
HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, CD_antigen: CD74 Gene name : CD74