RCHY1 antibody
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- Target See all RCHY1 Antibodies
- RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))
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Reactivity
- Human, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This RCHY1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- Purification
- purified
- Immunogen
- Purified recombinant fragment of human Pirh2 expressed in E. coli.
- Clone
- 1H10
- Isotype
- IgG1
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- Application Notes
- ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Ascitic fluid containing 0.03 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- 4°C, -20°C for long term storage
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Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3370-8, (2006) (PubMed).
: "Expression of Pirh2, a newly identified ubiquitin protein ligase, in lung cancer." in: Journal of the National Cancer Institute, Vol. 96, Issue 22, pp. 1718-21, (2004) (PubMed).
: "
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Differential response between the p53 ubiquitin-protein ligases Pirh2 and MdM2 following DNA damage in human cancer cells." in: Experimental cell research, Vol. 312, Issue 17, pp. 3370-8, (2006) (PubMed).
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- Target
- RCHY1 (Ring Finger and CHY Zinc Finger Domain Containing 1, E3 Ubiquitin Protein Ligase (RCHY1))
- Alternative Name
- Pirh2 (RCHY1 Products)
- Synonyms
- ARNIP antibody, CHIMP antibody, PIRH2 antibody, PRO1996 antibody, RNF199 antibody, ZNF363 antibody, Pirh2 antibody, Zfp363 antibody, Znf363 antibody, wu:fb37e07 antibody, zgc:101128 antibody, RCHY1 antibody, arnip antibody, chimp antibody, pirh2 antibody, harnip antibody, rnf199 antibody, znf363 antibody, pro1996 antibody, ring finger and CHY zinc finger domain containing 1 antibody, ring finger and CHY zinc finger domain containing 1 L homeolog antibody, RCHY1 antibody, Rchy1 antibody, rchy1 antibody, rchy1.L antibody
- Background
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Description: Pirh 2 (P53 induced RING-H2 protein), also known as RCHY1, it forms dimers through its N- and C-terminus in cells. The Pirh2 has ubiquitin-protein ligase activity and it binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. The Pirh2 is oncogenic because loss of p53 function contributes directly to malignant tumor development. Pirh2 expression decreases the level of p53, and a decrease of endogenous Pirh2 expression increases p53 levels. Pirh2 is therefore considered, together with MDM2, to act as a negative regulator of p53 function.
Aliases: ARNIP, CHIMP, RNF199, RCHY1
- Molecular Weight
- 30 kDa, 60 kDa (homodimer)
- Gene ID
- 25898
- HGNC
- 25898
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