PSMD8
Reactivity: Human, Mouse, Rat
WB, IF (p), IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1 : 1000 up to 1 : 5000 (AP staining) ICC: 1 : 200 IHC: not tested yet
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handling Advice
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Storage
-20 °C
Storage Comment
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
Nieradka, Ufer, Thiadens, Grech, Horos, van Coevorden-Hameete, van den Akker, Sofi, Kuhn, von Lindern: "Grsf1-induced translation of the SNARE protein Use1 is required for expansion of the erythroid compartment." in: PLoS ONE, Vol. 9, Issue 9, pp. e104631, (2014) (PubMed).
Takamori, Holt, Stenius, Lemke, Grønborg, Riedel, Urlaub, Schenck, Brügger, Ringler, Müller, Rammner, Gräter, Hub, De Groot, Mieskes, Moriyama, Klingauf, Grubmüller, Heuser, Wieland, Jahn: "Molecular anatomy of a trafficking organelle." in: Cell, Vol. 127, Issue 4, pp. 831-46, (2006) (PubMed).