MICA antibody

Catalog No. ABIN180912

This Mouse Monoclonal antibody specifically detects MICA in IP and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for MICA antibody (ABIN180912)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 6D4

Product Details anti-MICA Antibody

Specificity: This antibody is specific for nonclassical MHC class I chain A (MICA) and nonclassical MHC class I chain MICB (MICB).

Cross-Reactivity (Details): Also reacts to: MICB

Characteristics: Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

Purification: Affinity chromatography on Protein G

Immunogen: MICA transfected C1R cells. Spleen cells from immunised RBF/DnJ mice were fused withcells of the P3 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Immunoprecipitation. Flow Cytometry: use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA

Background: MICA and MICB are stress inducible antigens, which are closely related and appear functionally indistinguishable. MICA and MICB are ligands for NKG2D, an activating receptor on most natural killer (NK) cells, CD8 T cells and gamma delta T cells. MICA is principally expressed on intestinal epithelium, and several epithelial tumours. Expression may be induced to high surface levels by heat shock, oxidative stress, and virus infection. Clone 6D4 is reported to inhibit the cytotoxicity of NK cells stimulated by IFN alpha-treated dendritic cells.Synonyms: MHC class I polypeptide-related sequence A, MIC-A, PERB11.1

Gene ID: 4276

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response