TNFRSF1B
Reactivity: Human
WB
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Western Blot: 1.0 μg/mL - 2.0 μg/mL. Immunohistochemistry on frozen sections: 15 μg/mL - 25 μg/mL. ELISA: Using the concentrations stated above the detection limits are: ELISA - 5 ng/wellBLOT - 10 ng/lane (reducing conditions). Functional Assays: This antibody neutralises the biological effect of rhTNF RII,but not rhTNF RI. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing without preservatives or stabilizers
Preservative
Without preservative
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
TNFRSF1B
(Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor