Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhEotaxin-3 (100 ng/mL), a concentration of 5.0 - 7.0 μg/mL of this antibody is required. ELISA: Indirect: To detect hEotaxin-3 by indirect ELISA (using 100 μL/well antibody solution)a concentration of 0.5 - 2.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Sandwich: To detect hEotaxin-3 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL is required. In conjunction with Biotinylated Anti-HumanEotaxin-3 as a detection antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant hEotaxin-3. Western Blot: To detect hEotaxin-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hEotaxin-3 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1 - 1.0 mg/mL.
Buffer
PBS, pH 7.2
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Eotaxin 3 is a recently discovered CC chemokine. It binds to the CCR3 receptor and is highly chemotactic for eosinophils and basophils.Synonyms: C-C motif chemokine 26, CC chemokine IMAC, IMAC, MIP4 alpha, Macrophage inflammatory protein 4-alpha, SCYA26, Small-inducible cytokine A26, TSC-1, TSC1, Thymic stroma chemokine-1