V5 Epitope Tag antibody (FITC)

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Antigen
Reactivity
Tag
403
18
16
8
3
3
Host
Mouse
248
144
37
7
5
4
3
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
FITC
31
27
21
14
13
12
12
12
11
10
10
10
10
9
8
8
7
6
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF)
370
264
136
95
78
71
53
44
28
13
9
8
5
4
4
2
2
2
2
1
1
1
Options
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Immunogen Mice were infected with the paramyxovirus SV5, Simian-Virus 5. Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
Sequence GKPIPNPLLG LDST
Clone SV5-PK1
Isotype IgG2a
Purification Affinity chromatography on Protein G
Alternative Name Pk (V5) Epitope Tag (GKPIPNPLLGLDST)
Target Type Tag
Research Area Tags/Labels
Application Notes Immunofluorescence: 1/100, Membrane permeabilisation with 0.5 % IGEPAL is required for this application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product is manufactured under an exclusive license from the University of St. Andrews,UK.
Restrictions For Research Use only
Concentration 0.1 mg/mL
Buffer Containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Southern, Young, Heaney, Baumgärtner, Randall: "Identification of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics." in: The Journal of general virology, Vol. 72 ( Pt 7), pp. 1551-7, 1991 (PubMed).

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