C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody (Biotin)

Details for Product No. ABIN181893
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms CLEC7A, BGR, Clecsf12, beta-GR, CANDF4, CLECSF12, DECTIN1, Clec7b, RGD1565140
Reactivity
Mouse (Murine)
(60), (55), (20)
Host
Rat
(40), (34), (21)
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
(18), (6), (6), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Flow Cytometry (FACS)
(53), (26), (18), (12), (12), (10), (9), (6), (6), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN181893
709.50 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1. Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line.
Clone 2A11
Isotype IgG2b
Specificity This antibody recognises beta-glucan receptor, also known as Dectin-1. This antibody inhibits the binding of zymosan to macrophages via the beta-glucan receptor. The low endotoxin version (SM2025LE) is recommended for functional studies.
Purification Affinity chromatography on Protein G
Alternative Name CLEC7A / Dectin-1
Background Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells. As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Recent work has shown that Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.
Alternate names: BGR, Beta-glucan receptor, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, CLECSF12, DC-associated C-type lectin 1, DECTIN1, Dendritic cell- associated C-type lectin 1
Gene ID 56644
NCBI Accession NP_064392
UniProt Q6QLQ4
Application Notes Flow Cytometry: Neat - 1/10, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.1 mg/mL
Buffer PBS, pH 7.4 containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-C-Type Lectin Domain Family 7, Member A (CLEC7A) antibody (Biotin) Staining of mouse peripheral blood granulocytes with RAT ANTI MSE BETA GLUCAN RECEPTOR:BIOTIN (ABIN181893).
Background publications Taylor, Brown, Reid et al.: "The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 7, pp. 3876-82, 2002 (PubMed).

Underhill, Rossnagle, Lowell et al.: "Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production." in: Blood, Vol. 106, Issue 7, pp. 2543-50, 2005 (PubMed).

Validation Images
Did you look for something else?
back to top