Notch1 antibody
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- Target See all Notch1 (NOTCH1) Antibodies
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
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Reactivity
- Human, Mouse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Notch1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody is specific for Notch1, a single transmembrane receptor which is expressed in a range of cells including hematopoietic cells in mouse fetal liver, adult thymus and bone marrow.
- Purification
- Affinity Chromatography on Protein G
- Immunogen
- Synthetic peptide corresponding to cdc10-NCR region within mouse Notch1. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- MN1A
- Isotype
- IgG1
- Top Product
- Discover our top product NOTCH1 Primary Antibody
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- Application Notes
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Western Blot. Flow Cytometry: Membrane permeabilisation is required for this application. Immunohistochemistry on Frozen Sections (1/50-1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservataive.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- Notch1 (NOTCH1) (Notch 1 (NOTCH1))
- Alternative Name
- NOTCH1 (NOTCH1 Products)
- Synonyms
- notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody
- Background
- The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. Notch 1 is expressed in a range of cells including haematopoietic cells in mouse foetal liver, adult thymus and bone marrow. Notch 1 signalling plays a role in follicular differentiation, tissue homeostasis, and in both CD4+ and CD8+ cell maturation in the thymus. Studies have also implicated Notch 1 in the regulation of lymphopoieisis, myelopoiesis, and neurogenesis.Synonyms: Neurogenic locus notch homolog protein 1, Notch 1, TAN1, hN1
- Gene ID
- 4851
- NCBI Accession
- NP_060087
- UniProt
- P46531
- Pathways
- Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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