Early B-Cell Factor 3 (EBF3) (Middle Region) antibody

Details for Product No. ABIN182416
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Antigen
Synonyms EBF3, coe3, Xebf3, 3110018A08Rik, O/E-2, mKIAA4201, zgc:158829, Xcoe3, oe2, xebf-3, COE3, EBF-3, OE-2, AI790651, EB2, EB3
Epitope
Middle Region
(9), (3), (3), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Xenopus laevis, Human, Zebrafish (Danio rerio)
(23), (9), (7), (1), (1), (1)
Host
Rabbit
(15), (9), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(25), (15), (4), (1), (1)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN182416
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Immunogen Synthetic peptide directed towards the middle region of human EBF3
Sequence AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against EBF3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name EBF3
Background EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. Human EB1 family proteins, which include EB1, EBF3, and RP1, also associate with the tumor suppressor protein adenomatous polyposis coli (APC) and p150glued, a component of the dynactin complex.
Molecular Weight 60 kDa
Gene ID 253738
NCBI Accession NM_001005463, NP_001005463
UniProt Q9H4W6
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 551 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Early B-Cell Factor 3 (EBF3) (Middle Region) antibody WB Suggested Anti-EBF3 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:62500
Positive Control: Raji cell lysate
Background publications Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

General Bennett, Romigh, Eng: "Disruption of transforming growth factor-beta signaling by five frequently methylated genes leads to head and neck squamous cell carcinoma pathogenesis." in: Cancer research, Vol. 69, Issue 24, pp. 9301-5, 2009 (PubMed).

Validation Images
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