EBF3 antibody (Middle Region)

Catalog No. ABIN2777449

This Rabbit Polyclonal antibody specifically detects EBF3 in WB. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig and Rabbit.It has been mentioned in 1 publication

Quick Overview for EBF3 antibody (Middle Region) (ABIN2777449)

Target: EBF3 (Early B-Cell Factor 3 (EBF3))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBF3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-EBF3 Antibody

Binding Specificity: Middle Region

Purpose: EBF3 Antibody - middle region

Sequence: AHTGMMGVNS FSSQLAVNVS ETSQANDQVG YSRNTSSVSP RGYVPSSTPQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against EBF3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EBF3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099406

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-EBF3 antibody (ABIN2777449)

Bennett, Romigh, Eng: "Disruption of transforming growth factor-beta signaling by five frequently methylated genes leads to head and neck squamous cell carcinoma pathogenesis." in: Cancer research, Vol. 69, Issue 24, pp. 9301-5, (2009) (PubMed).

Target Details for EBF3

Target: EBF3 (Early B-Cell Factor 3 (EBF3))

Alternative Name: EBF3

Background:

Background Information: EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. Human EB1 family proteins, which include EB1, EBF3, and RP1, also associate with the tumor suppressor protein adenomatous polyposis coli (APC) and p150glued, a component of the dynactin complex.

Gene Name: Early B-cell factor 3

Alternative Symbols: COE3, OE-2, EBF-3, HADDS, O/E-2

Protein Name: Transcription factor COE3

Molecular Weight: 60kDa

Gene ID: 253738

NCBI Accession: NP_001005463

UniProt: Q9H4W6