ID4 antibody (N-Term)

Catalog No. ABIN183598

This Rabbit Polyclonal antibody specifically detects ID4 in WB. It exhibits reactivity toward Human, Mouse, Rat, Pig, Dog and Zebrafish (Danio rerio).It has been mentioned in 1 publication

Quick Overview for ID4 antibody (N-Term) (ABIN183598)

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Reactivity: Human, Mouse, Rat, Pig, Dog, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ID4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ID4 Antibody

Binding Specificity: N-Term

Purpose: ID4 Antibody - N-terminal region

Sequence: MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC

Predicted Reactivity: Dog: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ID4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ID4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP38058-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ID4 antibody (ABIN183598)

Melcher, Hartmann, Zopf, Herterich, Wilke, Müller, Rosler, Kudlich, Al-Taie, Rosenwald, Katzenberger, Scholtka, Seibold, Rogoll, Scheppach, Scheurlen, Lührs: "LOH and copy neutral LOH (cnLOH) act as alternative mechanism in sporadic colorectal cancers with chromosomal and microsatellite instability." in: Carcinogenesis, Vol. 32, Issue 4, pp. 636-42, (2011) (PubMed).

Target Details for ID4

Target: ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-Loop-Helix Protein (ID4))

Alternative Name: ID4

Background:

Background Information: Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995).[supplied by OMIM].

Gene Name: Inhibitor of DNA binding 4, dominant negative helix-loop-helix protein

Alternative Symbols: IDB4, bHLHb27

Protein Name: DNA-binding protein inhibitor ID-4

Molecular Weight: 17kDa

Gene ID: 3400

NCBI Accession: NP_001537

UniProt: P47928