Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody

Details for Product No. ABIN184024
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Antigen
Synonyms AA407051, AV149256, NRB54, P54NRB, nonA, nmt55, nrb54, p54nrb, xp54nrb, P54, NMT55
Epitope
C-Term
(6), (3), (3), (2), (1), (1), (1)
Reactivity
Dog (Canine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Xenopus laevis, Human
(33), (12), (11), (5), (2), (2), (1), (1), (1)
Host
Rabbit
(24), (9), (6)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(38), (14), (12), (8), (7), (5), (3), (2)
Pubmed 2 references available
Catalog no. ABIN184024
Quantity 100 µg
Price
229.00 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide directed towards the C terminal of human NONO
Sequence DGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGA EFAPNKRRRY
Predicted Reactivity Bovine : 100 %, Horse : 100 %, Human : 100 %, Rabbit : 100 %, Mouse : 90 %, Rat : 90 %, African clawed frog : 70 %
Characteristics This is a rabbit polyclonal antibody against NONO. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name NONO
Background NONO is DNA- and RNA binding protein, involved in several nuclear processes. It binds the conventional octamer sequence in double stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. It is involved in pre-mRNA splicing and interacts with U5 snRNA. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs, be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 and be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
Molecular Weight 52 kDa
Gene ID 4841
NCBI Accession NM_007363, NP_031389
UniProt Q15233
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 471 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody WB Suggested Anti-NONO Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody (2) anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody (Image 2)
anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody (3) anti-Non-POU Domain Containing, Octamer-Binding (NONO) (C-Term) antibody (Image 3)
Product cited in: Lazrek, Goffard, Schanen et al.: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006 (PubMed).

Background publications Talmud, Drenos, Shah et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006 (PubMed).

Hosts (24), (9), (6)
Reactivities (33), (12), (11), (5), (2), (2), (1), (1), (1)
Applications (38), (14), (12), (8), (7), (5), (3), (2)
Conjugates (1)
Epitopes (6), (3), (3), (2), (1), (1), (1)
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