E2F Transcription Factor 1 (E2F1) (C-Term) antibody

Details for Product No. ABIN184178
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Antigen
Synonyms
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)0 ... show more
CG6376, DRTF1/E2F, DmE2F-1, Dmel\\CG6376, Dp, E(Sev-CycE)3A, E(var)3-93E, E(var)3-95E, E(var)93E, E2F, E2F-1, E2F1, E2f1, Evar(3)164, dE2F, dE2F1, dE2f, dE2f1, de2f1, def21, drosE2F1, e2f, e2f1, l(3)07172, l(3)j3B1, l(3)j3C2, l(3)rM729, RBAP1, RBBP3, RBP3, mKIAA4009, xE2F, ATE2FB, E2F TRANSCRIPTION FACTOR-1 E2F1, E2F transcription factor 1, E2FB, E2FB TRANSCRIPTION FACTOR show less
Epitope
C-Term
(30), (24), (23), (17), (15), (11), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Cow (Bovine), Dog (Canine), Human
(270), (94), (58), (26), (26), (25), (12), (1), (1), (1), (1), (1)
Host
Rabbit
(221), (49), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(15), (12), (11), (6), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5)
Application
Western Blotting (WB)
(192), (80), (60), (48), (44), (41), (24), (24), (12), (8), (7), (5), (3), (2), (2), (1)
Pubmed 1 reference available
Quantity 50 μg
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Catalog No. ABIN184178
289.00 $
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Immunogen Synthetic peptide directed towards the C terminal of human E2F1
Sequence REDFSGLLPE EFISLSPPHE ALDYHFGLEE GEGIRDLFDC DFGDLTPLDF
Predicted Reactivity Dog : 100 %, Guinea pig : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rabbit : 100 %, Rat : 100 %
Characteristics This is a rabbit polyclonal antibody against E2F1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name E2F1
Background The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 47 kDa
Gene ID 1869
NCBI Accession NM_005225, NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-E2F Transcription Factor 1 (E2F1) (C-Term) antibody WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Daudi cell lysate
Background publications Unoki, Nishidate, Nakamura: "ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain." in: Oncogene, Vol. 23, Issue 46, pp. 7601-10, 2004 (PubMed).

Validation Images
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