ErbB2/Her2 antibody (pTyr1221, pTyr1222)

Catalog No. ABIN1842354

Product Details anti-ErbB2/Her2 Antibody

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Binding Specificity: pTyr1221, pTyr1222

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Rabbit Anti-HER2 (1,1222) Polyclonal Antibody detects endogenous levels of total HER2 protein.

Characteristics: Molecular Weight: Predicted Band Size 185 kDa, Observed Band Size 185 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from human HER2 around pTyr1221,pTyr1222 (N-L-YP-YP-W)

Isotype: IgG

Application Details

Application Notes: Western blot: 1:500-1:1,000,
IHC: 1:50-1:100,
IF: 1:100-1:200

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. It will remain stable for one year if stored properly.

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: HER2 (ErbB2/Her2 Products)

Synonyms: CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Erbb-2 antibody, Neu antibody, c-erbB2 antibody, c-neu antibody, mKIAA3023 antibody, wu:fv70f10 antibody, zgc:63601 antibody, erb2 antibody, erb-b2 receptor tyrosine kinase 2 antibody, ERBB2 antibody, Erbb2 antibody, erbb2 antibody

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development