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Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1) (C-Term) antibody

Details for Product No. ABIN184559
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Antigen
Synonyms sec61, sec61a, sec61alpha, SEC61A1, secY1, SEC61A, HSEC61, SEC61, AA408394, AA410007, Sec61a, chunp6898, fb62c11, sec61al1, wu:fb62c11
Epitope
C-Term
(6), (4), (2), (2)
Reactivity
Human, Mouse (Murine)
(16), (5), (5), (5), (3), (2), (1), (1)
Host
Goat
(12), (8), (1)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(18), (6), (4), (4), (4), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184559
374.27 $
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Immunogen Peptide with sequence C-KEQSEVGSMGALLF, from the C Terminus of the protein sequence according to NP_037468.1.
Cross-Reactivity Dog (Canine), Cow (Bovine), Pig (Porcine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Serum-free
Alternative Name SEC61A1
Background Synonyms: SEC61A1, HSEC61, SEC61, SEC61A, protein transport protein SEC61 alpha subunit isoform 1, sec61 homolog, Sec61 alpha 1 subunit (S. cerevisiae), Sec61 alpha 1 subunit, Sec61 alpha-1
Gene ID 29927
NCBI Accession NP_037468.1
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 48 kDa band observed in Human Brain (Cerebelum) lysates (calculated MW of 52.3 kDa according to NP_037468.1). Recommended concentration: 0.3-1 µg/mL.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Sec61 alpha 1 Subunit (S. Cerevisiae) (SEC61A1) (C-Term) antibody ABIN184559 staining (3µg/ml) of Daudi lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Görlich, Prehn, Hartmann et al.: "A mammalian homolog of SEC61p and SECYp is associated with ribosomes and nascent polypeptides during translocation." in: Cell, Vol. 71, Issue 3, pp. 489-503, 1992 (PubMed).

Validation Images
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