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B-Cell Linker (BLNK) (C-Term) antibody

Details for Product No. ABIN184602, Supplier: Login to see
Antigen
  • BLNK
  • blnk
  • MGC147045
  • BASH
  • Bca
  • Ly-57
  • Ly57
  • Lyw-57
  • SLP-65
  • AGM4
  • BLNK-S
  • LY57
  • SLP65
  • bca
Epitope
C-Term
24
24
18
18
13
13
13
12
10
8
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
182
92
59
4
4
4
3
1
1
Host
Goat
150
25
17
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
6
6
5
4
4
4
4
4
4
4
3
3
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
132
65
44
39
20
8
8
7
6
4
4
1
1
1
Supplier
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Immunogen Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.
Blocking Peptide Blocking peptide for this product available: ABIN369252
Specificity This antibody is expected to recognize both reported isoforms (NP_037446.1, NP_001107566.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BLNK / SLP-65 (BLNK Antibody Abstract)
Background Synonyms: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK-S, LY57, OTTHUMP00000020168
Gene ID 29760, 17060
NCBI Accession NP_001107566, NP_037446
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50 kDa band is observed. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-B-Cell Linker (BLNK) (C-Term) antibody (ABIN184602) ABIN184602 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lan...
Background publications Fu, Turck, Kurosaki et al.: "BLNK: a central linker protein in B cell activation." in: Immunity, Vol. 9, Issue 1, pp. 93-103, 1998 (PubMed).