B-Cell Linker (BLNK) (C-Term) antibody

Details for Product No. ABIN184602
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Antigen
Synonyms BLNK, blnk, MGC147045, BASH, Bca, Ly-57, Ly57, Lyw-57, SLP-65, AGM4, BLNK-S, LY57, SLP65, bca
Epitope
C-Term
(21), (20), (12), (10), (8), (6), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(125), (76), (52), (14), (14), (14), (1)
Host
Goat
(113), (10), (8)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(89), (46), (40), (34), (18), (8), (6), (5), (2), (1), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184602
374.27 $
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Immunogen Peptide with sequence C-KDSTRLKYAVKVS, from the C Terminus of the protein sequence according to NP_037446.1, NP_001107566.1.
Specificity This antibody is expected to recognize both reported isoforms (NP_037446.1, NP_001107566.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name BLNK / SLP-65
Background Synonyms: BLNK, SLP-65, B-cell linker, BASH, BLNK-s, Ly57, MGC111051, SLP65, B cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, OTTHUMP00000020167, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK-S, LY57, OTTHUMP00000020168
Gene ID 29760, 17060
NCBI Accession NP_001107566, NP_037446
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70 kDa band observed in lysates of cell line Daudi (calculated MW of 50.5 kDa according to NP_037446.1). The observed molecular weight corresponds to how SLP-65 is expected to run. However, when transiently expressed in HEK293, a 50 kDa band is observed. Recommended concentration: 1-3 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-B-Cell Linker (BLNK) (C-Term) antibody ABIN184602 staining (4µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Fu, Turck, Kurosaki et al.: "BLNK: a central linker protein in B cell activation." in: Immunity, Vol. 9, Issue 1, pp. 93-103, 1998 (PubMed).

Validation Images
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