PPP2R5D antibody (C-Term)
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- Target See all PPP2R5D Antibodies
- PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This PPP2R5D antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- PPP2R5D
- Sequence
- KRAEEFLTAS QEAL
- Specificity
- This antibody is expected to recognise reported isoforms (NP_006236.1 (iso 1), NP_851307.1, (iso 2) NP_851308.1) (iso 3) and NP_001257405.1 (iso 4).
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-KRAEEFLTASQEAL, from the C Terminus of the protein sequence according to NP_006236.1, NP_851307.1, NP_851308.1.
- Isotype
- IgG
- Top Product
- Discover our top product PPP2R5D Primary Antibody
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- Application Notes
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Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HepG2 and Jurkat, and in Human Cerebellum lysates, approx. 65-70 kDa in lysates of cell line NIH3T3, and approx.75 kDa in Rat Brain lysates, (calculated MW of 70 kDa according to Huma
Peptide ELISA: antibody detection limit dilution 1:20000.
- Comment
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- PPP2R5D (Protein Phosphatase 2, Regulatory Subunit B', delta (PPP2R5D))
- Alternative Name
- PPP2R5D (PPP2R5D Products)
- Synonyms
- B56D antibody, B'delta antibody, TEG-271 antibody, Tex271 antibody, zgc:73160 antibody, zgc:77529 antibody, ppp2r5d antibody, MDS026 antibody, protein phosphatase 2 regulatory subunit B'delta antibody, protein phosphatase 2, regulatory subunit B', delta antibody, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform antibody, protein phosphatase 2 regulatory subunit Bdelta antibody, PPP2R5D antibody, Ppp2r5d antibody, ppp2r5d antibody, LOC100176996 antibody, PPP2R2D antibody
- Background
- PPP2R5D, MGC2134, MGC8949, protein phosphatase 2, regulatory subunit B (B56), delta isoform, PP2A, B subunit, B' delta isoform, PP2A, B subunit, R5 delta isoform, PP2A, B subunit, B56 delta isoform, PP2A, B subunit, PR61 delta isoform, Serine/threonine pr
- Gene ID
- 5528
- NCBI Accession
- NP_006236, NP_851307, NP_851308
- Pathways
- PI3K-Akt Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades
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