Annexin A11 (ANXA11) antibody

Antigen: Annexin A11 (ANXA11)

Synonyms: ANX11, CAP50, Anx11, A830099O17Rik, ANXA11, MGC140235, anx11, cap50, MGC81584, anxa11, MGC89007, LOC100226075

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184685

Additional Information

Alternative name: Annexin A11

Gene ID: 311

Immunogen: Peptide with sequence YPGYPPPPGGYPP-C, from the N Terminus of the protein sequence according to NP_001148.1, NP_665875.1, NP_665876.1

Format: Affinity-purified

Description: All names and symbols: ANXA11, annexin A11, ANX11, CAP50, annexin XI, autoantigen, 56-kD, calcyclin-associated annexin 50, RP11-369J21, OTTHUMP00000019957, OTTHUMP00000019958,. Official Symbol: ANXA11. RefSeq number(s): NP_001148.1, NP_665875.1, NP_665876.1. Gene ontology terms: protein binding, calcium-dependent phospholipid binding, calcium ion binding, immune response, nuclear membrane, nucleoplasm, cytoplasm NP_001148.1, NP_665875.1 and NP_665876.1 are varients that encode the same protein..

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 55kDa band seen in HeLa lysate (Predicted MW of approx. 58kDa according to NP_001148.1). Recommended for use at 0.2-1µg/ml. Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of Basement Membrane in trophoblasts. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Research Area: Signaling

Restrictions: For Research Use only

Publications

Publications: Misaki, Pruijn, van der Kemp et al.: "The 56K autoantigen is identical to human annexin XI." in: The Journal of biological chemistry, Vol. 269, Issue 6, pp. 4240-6, 1994 (PubMed).