CPT1B antibody (C-Term)

Catalog No. ABIN184772

The Goat Polyclonal anti-CPT1B antibody has been validated for ELISA and IHC. It is suitable to detect CPT1B in samples from Human.

Quick Overview for CPT1B antibody (C-Term) (ABIN184772)

Target: CPT1B (Carnitine Palmitoyltransferase 1B (Muscle) (CPT1B))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CPT1B antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CPT1B Antibody

Binding Specificity: C-Term

Purpose: CPT1B (isoform 1)

Sequence: DIADLFQVPK AYS

Specificity: This antibody is expected to recognise human isoforms a (NP_689452.1, NP_689451.1, NP_004368.1, NP_001138609.1, NP_001138607.1), isoform c (NP_001138606.1) and isoform d (NP_001138608.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DIADLFQVPKAYS, from the C Terminus of the protein sequence according to NP_689452.1, NP_689451.1, NP_004368.1, NP_001138609.1, NP_001138607.1, NP_001138606.1, NP_001138608.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5 μg/mL.

Western Blot: Preliminary experiments gave no signal but low background in human kidney and HepG2 lysates at up to 1 μg/mL. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CPT1B

Target: CPT1B (Carnitine Palmitoyltransferase 1B (Muscle) (CPT1B))

Alternative Name: CPT1B

Background: CPT1B, carnitine palmitoyltransferase I, muscle, carnitine palmitoyltransferase 1B (muscle) , CPT1-M, M-CPT1, KIAA1670, CPTI, muscle, carnitine O-palmitoyltransferase I, mitochondrial muscle isoform, carnitine palmitoyltransferase 1A (muscle), carnitine p

Gene ID: 1375

NCBI Accession: NP_689452, NP_689451, NP_004368, NP_001138609, NP_001138607, NP_001138606, NP_001138608

Pathways: AMPK Signaling, Monocarboxylic Acid Catabolic Process