Claudin 14 (CLDN14) antibody

Antigen: Claudin 14 (CLDN14)

Synonyms: DFNB29, CLD1, SEMP1, ILVASC, AI851731, MGC109472, MGC81805, claudin-14, CLDN14

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184791

Additional Information

Alternative name: Claudin 14 / CLDN14

Gene ID: 23562, 56173 (mouse)

Immunogen: C-SATHSGYRLNDYV

Description: All names and symbols: CLDN14, claudin 14, DFNB29. Official Symbol: CLDN14. RefSeq number(s): NP_036262, NP_652763. Gene ontology terms: protein complex assembly

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 23kDa band observed in human liver lysate (calculated MW of 25.7kDa according to NP_652763). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184791 (1µg/ml) staining of human liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Wilcox, Burton, Naz et al.: "Mutations in the gene encoding tight junction claudin-14 cause autosomal recessive deafness DFNB29." in: Cell, Vol. 104, Issue 1, pp. 165-72, 2001 (PubMed).