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MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (C-Term) antibody

Details for Product No. ABIN184822, Supplier: Log in to see
Antigen
  • cb408
  • zgc:112133
  • MAD2L1
  • mad2
  • XMad2
  • AA673185
  • MAD2
  • HSMAD2
Epitope
C-Term
15
8
5
5
3
3
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
104
29
26
4
4
4
2
2
1
1
Host
Goat
68
23
13
1
Clonality
Polyclonal
Conjugate
Un-conjugated
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
89
38
21
18
18
17
11
3
2
1
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-KVNSMVAYKIPVND, from the C Terminus of the protein sequence according to NP_002349.1.
Blocking Peptide Blocking peptide for this product available: ABIN369385
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MAD2L1 (MAD2L1 Antibody Abstract)
Background Synonyms: MAD2L1, MAD2 mitotic arrest deficient-like 1 (yeast), MAD2, HSMAD2, mitotic arrest deficient, yeast, homolog-like 1, MAD2 (mitotic arrest deficient, yeast, homolog)-like 1, MAD2-like 1, MAD2-like protein 1
Gene ID 4085, 56150
NCBI Accession NP_002349
Research Area Cell Cycle
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 24 kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5 kDa according to NP_002349.1). Recommended concentration: 2-4 µg/mL. An additional band of unknown identity was also consistently observed at approx. 29 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Li, Benezra: "Identification of a human mitotic checkpoint gene: hsMAD2." in: Science (New York, N.Y.), Vol. 274, Issue 5285, pp. 246-8, 1996 (PubMed).