Karyopherin alpha 4 (Importin alpha 3) (KPNA4) antibody

Antigen: Karyopherin alpha 4 (Importin alpha 3) (KPNA4)

Synonyms: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP1, SRP3, IPOA3, MGC12217, MGC26703, 0335/13, IMPalpha3, Imp alpha-3, Kap alpha3, Kpna3, dKap-alpha3, imp alpha3, imp-a3, imp-alpha3, l(3)S033513, DmelCG9423, CG9423, 6, ATIMPALPHA3, IMPA-3, IMPORTIN ALPHA 3, IMPORTIN ALPHA ISOFORM 3, MODIFIER OF SNC1, MOS6, T10M13.16, T10M13_16, LOC100224013

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185160

Additional Information

Alternative name: KPNA4 / IPOA3

Gene ID: 3840

Immunogen: C-NSSANVPTEGFQF

Format: Affinity-purified

Description: All names and symbols: KPNA4, IPOA3, QIP1, SRP3, MGC12217, MGC26703, karyopherin alpha 4 (importin alpha 3), importin alpha 3, importin-alpha-Q1, karyopherin alpha 4. Official Symbol: KPNA4. RefSeq number(s): NP_002259.1. Gene ontology terms: NLS-bearing substrate-nucleus import, importin, alpha-subunit

Specificity: Human, Mouse, Rat, Dog

Synonyms: SRP1, SRP4, IPOA4, hSRP1, SRP1gamma, QIP1, SRP3, IPOA3, MGC12217, MGC26703, 0335/13, IMPalpha3, Imp alpha-3, Kap alpha3, Kpna3, dKap-alpha3, imp alpha3, imp-a3, imp-alpha3, l(3)S033513, DmelCG9423, CG9423, 6, ATIMPALPHA3, IMPA-3, IMPORTIN ALPHA 3, IMPORTIN ALPHA ISOFORM 3, MODIFIER OF SNC1, MOS6, T10M13.16, T10M13_16, LOC100224013

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1/32,000. Western Blot: Approx 55-60kDa band observed in Human Testis and Ovary lysates (predicted size of approx. 58kDa according to NP_002259.1). Recommended for use at 0.2-1µg/ml Immunohistochemistry: In paraffin embedded Human Testis, shows strong nuclear staining of some cells in the seminiferous tubules. Recommended concentration, 3µg/ml. Immunohistochemistry: In paraffin embedded Human Small Intestine shows pixulate staining in nuclei of enterocytes. Recommended concentration, 1-2µg/ml. Figure 2: ABIN185160 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185160 staining (0.5µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Figure 1: ABIN185160 (3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. Figure 2: see Application Notes.

anti-Karyopherin alpha 4 (Importin alpha 3) (KPNA4) antibody (Image 3)

Publications

Publications: Seki, Tada, Katada et al.: "Cloning of a cDNA encoding a novel importin-alpha homologue, Qip1: discrimination of Qip1 and Rch1 from hSrp1 by their ability to interact with DNA helicase Q1/RecQL." in: Biochemical and biophysical research communications, Vol. 234, Issue 1, pp. 48-53, 1997 (PubMed).