Lck Interacting Transmembrane Adaptor 1 (LIME1) antibody

Antigen: Lck Interacting Transmembrane Adaptor 1 (LIME1)

Synonyms: LIME, LP8067, FLJ20406, dJ583P15.4, RP4-583P15.5, 2810038K19Rik, RGD1562795, LIME1, DKFZp459A158

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185246

Additional Information

Alternative name: Lime1 (mouse)

Gene ID: 72699 (mouse)

Immunogen: C-ENVYESIKEMGL

Format: Affinity-purified

Description: All names and symbols: Lime1, Lck interacting transmembrane adaptor 1, 2810038K19Rik, LIME, RIKEN cDNA 2810038K19 gene. Official Symbol: Lime1. RefSeq number(s): NP_076173.1. Gene ontology terms: integral to membrane, extracellular space

Specificity: Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 32kDa band observed in Mouse Heart lysates (predicted MW of 31kDa according to NP_076173.1). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185246 staining (1µg/ml) of Mouse Heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hur, Son, Lee et al.: "LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1463-73, 2003 (PubMed).