Forkhead Box O3 (FOXO3) antibody

Antigen: Forkhead Box O3 (FOXO3)

Synonyms: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, Fkhr2, C76856, Foxo3a, 1110048B16Rik, 2010203A17Rik, Fkhrl1, FoxO3, FoxO3A, FOXO3, zgc:92176, wu:fb50g02, wu:fi33f07, foxo3, foxo3l, xFox06, foxO3, foxO

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185248

Additional Information

Alternative name: FOXO3A / FKHRL1

Gene ID: 2309, 56484 (mouse)

Immunogen: C-GAKQASSQSWVPG

Description: All names and symbols: FOXO3A, FKHRL1, AF6q21, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, forkhead box O3A, forkhead (Drosophila) homolog (rhabdomyosarcoma) like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1. Official Symbol: FOXO3A. RefSeq number(s): NP_001446.1, NP_963853.1. Gene ontology terms: transcription factor activity, induction of apoptosis, cell growth and/or maintenance, regulation of transcription, DNA-dependent, apoptosis, transcription from Pol II promoter, cytoplasm, nucleus

Specificity: Both variants represent identical protein (NP_001446.1and NP_963853.1). Human, Mouse, Rat, Dog 673 aa

Synonyms: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, Fkhr2, C76856, Foxo3a, 1110048B16Rik, 2010203A17Rik, Fkhrl1, FoxO3, FoxO3A, FOXO3, zgc:92176, wu:fb50g02, wu:fi33f07, foxo3, foxo3l, xFox06, foxO3, foxO

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 95kDa band observed in Human Brain and Heart lysates (predicted MW of 77kDa according to NP_001446). Recommended for use at 0.5-2 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185248 staining (0.5µg/ml) of Human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Brunet, Bonni, Zigmond et al.: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999 (PubMed).